SUMOtag

In contrast to ubiquitin, SUMO is not used to tag proteins for degradation. Mature SUMO is produced when the last four amino acids of the C-terminus have ...

Our SUMO-tag expression system maximizes the yield of soluble, functional proteins in E. coli. SUMO functions as both a chaperonin and initiator of protein ...

由 T Panavas 著作 · 2009 · 被引用 200 次 — In eukaryotic cells, the reversible attachment of small ubiquitin-like modifier (SUMO) protein is a post-translational modification that has been ...

由 D Kuo 著作 · 2014 · 被引用 41 次 — SUMO as a solubility tag and in vivo cleavage of SUMO fusion proteins with Ulp1. Methods Mol Biol. 2014:1177:71-80. doi: 10.1007/978-1-4939-1034-2_6.

SUMO標籤可作為重組蛋白表達的融合標籤和分子伴侶，以增加重組蛋白的表達量和溶解度。 SUMO蛋白酶在體外可以高效去除SUMO標籤，有利於產生具有天然N端的靶蛋白。

SUMO tag, as a fusion partner in various expression systems,was found to enhance recombinant protein yieldwith significant improvements of stability and ...

Specifications · Species of Origin. Human · Affinity Tag. His-SUMO Tag (N-term) · Purity. >98% as determined by SDS-PAGE analysis. · Endotoxin level.

The purified antibody is immobilized at 3-5 mg antibody per mL of 50% slurry and this kit allows a rapid and efficient affinity purification of active SUMO ...

SUMO proteins are small acidic proteins with distant homology to ubiquitin. At primary amino acid sequence, they are 10–20% identical to ubiquitin.